It also accommodates the changes at the NCBI BLAST server and fixes to reported problems. The upgrade offers compatibility with Snow Leopard, Mac OS X 10.6. Pre-designed or published primers can also be analyzed. The primers designed can be BLAST searched against nucleotide databases at NCBI to check their specificity. The HRMA primers are designed avoiding template secondary structures, assuring efficient primer extension. The primers are designed flanking a SNP of interest to generate the shortest possible amplicons with detectable melting temperature variation. Beacon Designer™ 7.70 Releasedīeacon Designer can now design primers for High Resolution Melting Analysis (HRMA). Users can now choose the regions of homologies to avoid by providing setting the percent identity and e-value. The upgrade accommodates the most recent changes at the BLAST server, including support for the latest genomic databases. For SYBR® Green assays, the program checks if either of the two primers span the SNP to be detected. Similarly, given a forward or reverse SNP flanking primer, a compatible primer and TaqMan® probe are designed. Given a wild or mutant TaqMan® probe, the program first checks if it is designed to detect the SNP selected and proceeds to design compatible SNP flanking primers. The design parameters have now been optimized, based on empirical and theoretical evidence, to improve the success in a multiplex reaction.īeacon Designer™ now offers improved SNP assay evaluation. Parameters such as the GC%, Avoiding G base at the 5' end of the probe, avoiding more Gs than Cs in the probe, avoiding T base at the 3' end of the primer are settable to ensure an efficient design.ĭesigning multiplex assays is a lot easier. Beacon Designer™ 7.80 Releasedīeacon Designer™ now enables better control over TaqMan design. Projects created in Beacon Designer can be exported in a zipped format for easy collaboration. Similarly, if the search results are sorted taking into consideration the probe rating, the assay with best rated probe is displayed. If the search results are sorted on the basis of primer rating, the assay with the best sense/anti-sense rating is displayed. Additionally, the oligos designed for the five sequences are checked by the program for cross hybridization and their multiplexing ability.Īssays designed can now be sorted on either primer or probe rating. Multiplex assays are now supported for five sequences fully utilizing all the five real time PCR cycler channels. The program reports amplicons and their melting temperatures for both the wild as well as the mutant alleles. Beacon Designer™ 7.90 Releasedīeacon Designer now supports HRMA primer design for all mutation types including deletions, insertions, substitutions and mixed mutations, along with SNPs. The upgrade accommodates the changes at the NCBI BLAST server, the latest databases available for BLAST and fixes to reported problems. The latest changes at the NCBI BLAST, including latest genomic databases and fixes to reported problems are also accommodated. The upgrade offers compatibility with Lion, Mac OS X 10.7. It would not only make the design easy and convenient for large number of sequences but also facilitate in saving time for your research. The saved template of parameters can be called for future searches. Primer and probe design parameters can now be saved as a template for repeated use. The primers are designed such that at least one of the primers from the pair spans one of the junctions selected by the user. Beacon Designer™ 8.00 Releasedīeacon Designer now enables primer design across exon-exon and exon-intron junctions to selectively amplify cDNA from a mixture. The upgrade offers compatibility with Windows 64bit OS. The version is now compatible with Windows 8. The upgrade accommodates the latest genomic databases available at the NCBI BLAST server and fixes to reported problems. The upgrade offers compatibility with Mavericks, Mac OS X 10.9. Latest changes to the genomic databases at the NCBI-BLAST along with fixes to reported problems are accommodated. The upgrade accommodates the latest genomic databases available at the NCBI BLAST server and fixes to reported issues. Beacon Designer 8.20 accommodates these changes. Beacon Designer™ 8.20 ReleasedĪs per the US government mandate, NCBI has made changes on its web sites and services to improve the security and privacy of the user. The upgrade accommodates the latest genomic databases available at the NCBI-BLAST server and the recent changes made by NCBI on its web sites and services to improve security and privacy. Beacon Designer™ Upgrades Beacon Designer™ 8.21 Released
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